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ACTOne™ cAMP Fluorimetric ELISA Assay Kit, ten 96-well plates (CB-80500-513)
Adenosine 3, 5 cyclic monophosphate (cAMP) is an important second messenger in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of GPCRs. ACTOne cAMP Fluorimetric ELISA Assay Kit is based on the competition between HRP-labeled cAMP and free cAMP for a fixed number of cAMP antibody binding sites. In the absence of free cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. In the presence of free cAMP, HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex. ACTOne cAMP Florimetric ELISA Assay Kit provides the sensitive method for detecting cAMP level in biochemical or cell-based assay system. Compared to other ELISA cAMP assay kits, our kit eliminates the tedious acetylation step. The kit uses Amplite Red as a fluorimetric substrate to quantify the HRP activity. The assay can be performed in a convenient 96-well or 384- well microtiter-plate format and easily adapted to automation. The fluorescent product formed is proportional to the activity of HRPcAMP conjugate. Kit Components Amount Component A: cAMP Standard 1 vial (33 g) Component B: Assay Buffer 500 mL Component C: HRP-cAMP Conjugate 1 vial Component D: 10X Wash Solution 2x100 mL Component E: Cell Lysis Buffer 100 mL Component F: 3% H 2 O 2 50 L Component G: Amplite Red 1 vial Component H: Anti-cAMP Ab Coated 96-Well Plate 10 plates Component I: Substrate Buffer 2x100 mL Note: Do not freeze Anti-cAMP Ab Pre-coated 96-well plate (Component H), store it at 4 o C. Datasheet
Frequently Asked Questions
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Why is IntelixBio LLC so popular?
IntelixBio has swiftly become a household name in the medical industry, primarily owing to its cutting-edge medical lab equipment. Our commitment to excellence resonates through every facet of our products, ensuring that laboratories worldwide receive top-tier solutions for their diagnostic needs. Our medical lab equipment is engineered with precision and innovation, embodying the pinnacle of technological advancement in the field. From state-of-the-art analyzers to automated specimen-handling systems, our extensive catalog caters to diverse laboratory requirements, fostering efficiency and accuracy in every diagnostic process. Laboratories entrust IntelixBio's medical lab equipment for its unwavering reliability, empowering healthcare professionals with the tools they need to deliver optimal patient care.
At IntelixBio, our dedication to advancing healthcare is exemplified through our unparalleled range of medical lab equipment. Each product is meticulously crafted to meet the exacting standards of modern laboratory environments, facilitating seamless integration and operation. Our commitment to quality permeates every aspect of our offerings, ensuring that laboratories can consistently rely on our medical lab equipment to deliver precise and dependable results. With a comprehensive selection that spans from basic laboratory essentials to sophisticated diagnostic instruments, IntelixBio remains the foremost choice for institutions seeking excellence in medical equipment solutions. Trust IntelixBio to elevate your laboratory capabilities with our innovative medical lab equipment, empowering you to drive progress in healthcare delivery.
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What products do you offer?
At IntelixBio LLC, we offer high-quality medical lab equipment. Our lab supplies include:
- Protective gear
- Antibody immunodetection supplies
- Bioreagents
- Lab Supplies
- Immunohistochemistry Tools
- Instruments and Equipment
- PPE
- Sample preparation supplies
Visit our online shop to view our available medical lab equipment and purchase the items that match your unique styles and needs today.
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Where do you ship your medical lab equipment?
We ship our medical lab equipment to customers nationwide. Browse our online shop to view our impressive inventory of available lab equipment today and purchase the products that meet your unique style and needs. We can't wait to prove why Intelixbio.com is one of the nation's best medical lab supply providers.
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How can I contact your company?
You can reach IntelixBio LLC by phone at 240-316-3161, by email at info@intelixbio.com, or by filling out one of the contact forms on our website.
When you contact our team, you can expect fast, friendly, and professional assistance with all your medical lab equipment needs.
