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AbinScience InVivoMAb Anti-Human R-Spondin 3 Antibody (Iv0181) (HV514010-1MG)
Overview
| Catalog No. | HV514010-1MG |
|---|---|
| Description | AbinScience InVivoMAb Anti-Human RSPO3 Antibody (Iv0181)[Iv0181] (HV514010-1MG) is a human monoclonal
antibody detecting RSPO3
in ELISA, FCM, Neutralization. Suitable for Human.
HIGHLIGHTS
|
| Species reactivity | Human |
| Applications | ELISA, FCM, Neutralization |
| Host species | Human |
| Isotype | IgG1, kappa |
| Clone ID | Iv0181 |
| Clonality | Monoclonal |
| Target | RSPO3, hPWTSR, hRspo3, PWTSR, R-spondin-3, Thrombospondin type-1 domain-containing protein 2, Protein with TSP type-1 repeat, Roof plate-specific spondin-3, THSD2 |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | Q9BXY4 |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Background | R-spondin-3 (RSPO3) is a ~30 kDa protein. Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. 1. de Lau, W. et al. (2011) Nature 476, 293-7. PMID: 21727895
2. Glinka, A. et al. (2011) EMBO reports 12, 1055-61. PMID: 21909076
3. Gong, X. et al. (2012) PloS one 7, e37137. PMID: 22615920
4. Szenker-Ravi, E. et al. (2018) Nature 557, 564-569. PMID: 29769720
|
| Note | For research use only. Not suitable for clinical or therapeutic use. |
Images
Flow-cytometry using anti-human RSPO3 antibody.RSPO3 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human RSPO3 antibody monoclonal antibody (Catalog # HV514010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for InVivoMAb Anti-Human RSPO3 Antibody (Iv0181).