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Abinscience Human TREM2 Recombinant Protein (N-His-SUMO) (HV166032)
Overview
| Catalog No. | HV166032 |
|---|---|
| Description | Abinscience Recombinant Human TREM2 Protein, N-His-SUMO(HV166032) expressed in E. coli, spanning His19-Ser174. Purity: >90%
by SDS-PAGE.
HIGHLIGHTS
|
| Expression system | E. coli |
| Accession | Q9NZC2 |
| Protein length | His19-Ser174 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Homo sapiens (Human) |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Predicted molecular weight | 29.66 kDa |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | Triggering receptor expressed on myeloid cells 2, Triggering receptor expressed on monocytes 2, TREM-2, TREM2 |
| Background | Triggering receptor expressed on myeloid cells 2 (TREM2) is a ~25 kDa protein. Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding. Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia. Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1. Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia. Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin. 1. Bouchon, A. et al. (2000) Journal of immunology (Baltimore, Md. : 1950) 164, 4991-5. PMID: 10799849
2. Yeh, FL. et al. (2016) Neuron 91, 328-40. PMID: 27477018
3. Zhao, Y. et al. (2018) Neuron 97, 1023-1031.e7. PMID: 29518356
4. Sudom, A. et al. (2018) The Journal of biological chemistry 293, 12634-12646. PMID: 29794134
5. Kleinberger, G. et al. (2014) Science translational medicine 6, 243ra86. PMID: 24990881
6. Filipello, F. et al. (2018) Immunity 48, 979-991.e8. PMID: 29752066
7. Bouchon, A. et al. (2001) The Journal of experimental medicine 194, 1111-22. PMID: 11602640
8. Paloneva, J. et al. (2003) The Journal of experimental medicine 198, 669-75. PMID: 12925681
|
| Note | For research use only. |
Images
SDS-PAGE for Recombinant Human TREM2 Protein, N-His-SUMO
Detected by Research Grade Anti-Human TREM2 Antibody (AL2p-58) (Catalog No: HV166026) in indirect ELISA
Detected by Research Grade Anti-Human TERM2 Antibody (DNL919-ATV) (Catalog No: HV166076) in indirect ELISA
Detected by Iluzanebart (Catalog No: HV166066) in indirect ELISA.
Datasheets
Human TREM2 Recombinant Protein (N-His-SUMO).pdf