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Abinscience Human Tau Recombinant Protein (C-His) (HY086012)
Overview
| Catalog No. | HY086012 |
|---|---|
| Description | Abinscience Recombinant Human MAPT/Tau/PHF-tau Protein, C-His(HY086012) expressed in E. coli, spanning Met1-Leu758. Purity: >90%
by SDS-PAGE.
HIGHLIGHTS
|
| Expression system | E. coli |
| Accession | P10636 |
| Protein length | Met1-Leu758 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Homo sapiens (Human) |
| Nature | Recombinant |
| Tested applications | WB |
| Target | MAPT, MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau, TAU |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Predicted molecular weight | 80.23 kDa |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | MAPT, MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau, TAU |
| Background | Microtubule-associated protein tau (MAPT) is a ~78 kDa protein. Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. 1. Yoshida, H. et al. (2012) Journal of neurochemistry 120, 165-76. PMID: 21985311
2. Sandberg, A. et al. (2020) Neurobiology of disease 146, 105079. PMID: 32961270
|
| Note | For research use only. |
Images
SDS-PAGE for Recombinant Human MAPT/Tau/PHF-tau Protein, C-His
Detects Gosuranemab in indirect ELISAs.
Detects Zagotenemab in indirect ELISAs.
Detects Tilavonemab in indirect ELISAs.
Detected by Anti-MAPT/Tau/PHF-tau monoclonal antibody (Catalog No: HY086013)
Detected by Research Grade Anti-Human MAPT/Tau/PHF-tau (DC8E8) (Catalog No: HY086013) in indirect ELISA
Detected by 2C5 (Catalog No: HY086243) in indirect ELISA.
Detected by SAA2138 (Catalog No: HY086253) in indirect ELISA.