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Abinscience InVivoMAb Anti-Clostridium botulinum botA/BoNT Antibody (BNTA-9.1) (JN888010-50ug)
Overview
| Catalog No. | JN888010-50ug |
|---|---|
| Description | Abinscience InVivoMAb Anti-Clostridium botulinum botA/BoNT Antibody (BNTA-9.1)[BNTA-9.1] (JN888010-50ug) is a human monoclonal
antibody detecting Botulinum neurotoxin type A
in ELISA, Neutralization.
|
| Species reactivity | Clostridium botulinum |
| Applications | ELISA, Neutralization |
| Host species | Human |
| Isotype | IgG1 |
| Clone ID | BNTA-9.1 |
| Clonality | Monoclonal |
| Target | Botulinum neurotoxin type A, BoNT/A, Bontoxilysin-A, BOTOX, Botulinum neurotoxin type A1, Botulinum neurotoxin A light chain, LC, 3.4.24.69, Botulinum neurotoxin A heavy chain, HC, botA, atx, bonT |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Associated products | pH 7.4 Dilution Buffer |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P0DPI0 |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Background | Botulinum neurotoxin type A (botA/BoNT) is a ~149 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol. 1. BURGEN, AS. et al. (1949) The Journal of physiology 109, 10-24. PMID: 15394302
2. Zhou, L. et al. (1995) Biochemistry 34, 15175-81. PMID: 7578132
3. Fischer, A. et al. (2007) The Journal of biological chemistry 282, 29604-11. PMID: 17666397
4. Fischer, A. et al. (2008) PLoS pathogens 4, e1000245. PMID: 19096517
5. Yowler, BC. et al. (2002) The Journal of biological chemistry 277, 32815-9. PMID: 12089155
6. Dong, M. et al. (2006) Science (New York, N.Y.) 312, 592-6. PMID: 16543415
7. Mahrhold, S. et al. (2006) FEBS letters 580, 2011-4. PMID: 16545378
8. Dong, M. et al. (2008) Molecular biology of the cell 19, 5226-37. PMID: 18815274
|
| Note | For research use only. Not suitable for clinical or therapeutic use. |
Images
SDS-PAGE for InVivoMAb Anti-Clostridium botulinum botA/BoNT Antibody (BNTA-9.1).
Datasheets
InVivoMAb Anti-Clostridium botulinum botA/BoNT Antibody (BNTA-9.1).pdf