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Abinscience InVivoMAb Anti-Human ACE-2 Antibody (3E8#) (HV157020-50ug)
Overview
| Catalog No. | HV157020-50ug |
|---|---|
| Description | Abinscience InVivoMAb Anti-Human ACE2 Antibody (3E8#)[3E8#] (HV157020-50ug) is a mouse monoclonal
antibody detecting Angiotensin-converting enzyme homolog
in ELISA, FCM, Neutralization. Suitable for Human.
HIGHLIGHTS
|
| Species reactivity | Human |
| Applications | ELISA, FCM, Neutralization |
| Host species | Mouse |
| Isotype | IgG2a, kappa |
| Clone ID | 3E8# |
| Clonality | Monoclonal |
| Target | Angiotensin-converting enzyme homolog, ACE2, ACEH, Metalloprotease MPROT15, Angiotensin-converting enzyme-related carboxypeptidase, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | Q9BYF1 |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Background | Angiotensin-converting enzyme 2 (ACE2) is a ~92 kDa protein. Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency. In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity. 1. Wang, W. et al. (2016) Hypertension (Dallas, Tex. : 1979) 68, 365-77. PMID: 27217402
2. Tipnis, SR. et al. (2000) The Journal of biological chemistry 275, 33238-43. PMID: 10924499
3. Donoghue, M. et al. (2000) Circulation research 87, E1-9. PMID: 10969042
4. Vickers, C. et al. (2002) The Journal of biological chemistry 277, 14838-43. PMID: 11815627
5. Zisman, LS. et al. (2003) Circulation 108, 1707-12. PMID: 14504186
6. Rushworth, CA. et al. (2008) The FEBS journal 275, 6033-42. PMID: 19021774
8. Kowalczuk, S. et al. (2008) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22, 2880-7. PMID: 18424768
9. Camargo, SM. et al. (2009) Gastroenterology 136, 872-82. PMID: 19185582
|
| Note | For research use only. Not suitable for clinical or therapeutic use. |
Images
SDS-PAGE for InVivoMAb Anti-Human ACE2 Antibody (3E8#).
Detects recombinant human ACE2 Protein, No tag (Catalog No: HV157011) in indirect ELISA
Flow-cytometry APC using anti-human ACE2 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human ACE2 monoclonal antibody (Catalog HV157020) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry PE using anti-human ACE2 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human ACE2 monoclonal antibody (Catalog HV157020) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry FITC using anti-human ACE2 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human ACE2 monoclonal antibody (Catalog HV157020) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot analysis was performed using anti-/ monoclonal antibody at 1ug/mL on various samples.
Lane 1: ATGE2061 200ng
Lane 2: 1* loading buffer
Datasheets
InVivoMAb Anti-Human ACE-2 Antibody (3E8#).pdf