Hec1 Polyclonal Antibody, 100µg, (ATB-T2121)

Background: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.Product datasheet: Overview Product Description Hec1 Polyclonal Antibody, 100µg, (ATB-T2121) Species Reactivities Human,Mouse Immunogen Synthesized peptide derived from the Internal region of human Hec1. Properties Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage Instructions-20°C/1 year Clonality Polyclonal References: HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis. Chen Y., Riley D.J., Chen P.-L., Lee W.-H.Mol. Cell. Biol. 17:6049-6056(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Lymph. HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins. Chen Y., Sharp Z.D., Lee W.-H.J. Biol. Chem. 272:24081-24087(1997) [PubMed] [Europe PMC] Cited for: INTERACTION WITH NEK2; PSMC2; PSMC5 AND SMC1A, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins. Zheng L., Chen Y., Lee W.-H.Mol. Cell. Biol. 19:5417-5428(1999) [PubMed] [Europe PMC] Cited for: INTERACTION WITH NEK2; PSMC2; PSMC5; RB1 AND SMC1A. Retinoblastoma protein enhances the fidelity of chromosome segregation mediated by hsHec1p. Zheng L., Chen Y., Riley D.J., Chen P.-L., Lee W.-H.Mol. Cell. Biol. 20:3529-3537(2000) [PubMed] [Europe PMC] Cited for: INTERACTION WITH RB1 AND SMC1A, MUTAGENESIS OF GLU-234. Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation. Chen Y., Riley D.J., Zheng L., Chen P.-L., Lee W.-H.J. Biol. Chem. 277:49408-49416(2002) [PubMed] [Europe PMC] Cited for: INTERACTION WITH NEK2, PHOSPHORYLATION AT SER-165 BY NEK2. Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2. Martin-Lluesma S., Stucke V.M., Nigg E.A.Science 297:2267-2270(2002) [PubMed] [Europe PMC] Cited for: FUNCTION, SUBCELLULAR LOCATION. Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores. DeLuca J.G., Howell B.J., Canman J.C., Hickey J.M., Fang G., Salmon E.D.Curr. Biol. 13:2103-2109(2003) [PubMed] [Europe PMC] Cited for: FUNCTION, SUBCELLULAR LOCATION. Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1. Stucke V.M., Baumann C., Nigg E.A.Chromosoma 113:1-15(2004) [PubMed] [Europe PMC] Cited for: FUNCTION. The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo. Joseph J., Liu S.-T., Jablonski S.A., Yen T.J., Dasso M.Curr. Biol. 14:611-617(2004) [PubMed] [Europe PMC] Cited for: FUNCTION.