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Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit, 96 Tests, E-EL-H6126-96T
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Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit, 96 Tests, E-EL-H6126-96T

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Product Details

Elabscience® Human NT-proBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit, E-EL-H6126-96T

Product Summary

Sensitivity 0.09 ng/mL
Detection Range 0.16-10 ng/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Human
Specificity This kit recognizes Human NT-proBNP in samples.No significant cross-reactivity or interference between Human NT-proBNP and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NT-proBNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NT-proBNP. You can calculate the concentration of Human NT-proBNP in the samples by comparing the OD of the samples to the standard curve.

Background :

Uniport ID P16860
Research Area Cancer , Cardiovascular , Neuroscience
Citations:


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Human NT-proBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit, 96 Tests, E-EL-H6126-96T
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