Neddylin Polyclonal Antibody, 100µg, (ATB-T3026)

Background: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Product datasheet: Overview Product Description Neddylin Polyclonal Antibody, 100µg, (ATB-T3026) Species Reactivities Human,Mouse,Rat,Monkey Immunogen Synthesized peptide derived from the N-terminal region of human Neddylin. Properties Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage Instructions-20°C/1 year Clonality Polyclonal References: A new NEDD8-ligating system for cullin-4A. Osaka F., Kawasaki H., Aida N., Saeki M., Chiba T., Kawashima S., Tanaka K., Kato S.Genes Dev. 12:2263-2268(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT. Tissue: Fibrosarcoma. Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201). Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Conjugation of the ubiquitin-like protein NEDD8 to cullin-2 is linked to von Hippel-Lindau tumor suppressor function. Liakopoulos D., Buesgen T., Brychzy A., Jentsch S., Pause A.Proc. Natl. Acad. Sci. U.S.A. 96:5510-5515(1999) [PubMed] [Europe PMC] Cited for: PROTEIN SEQUENCE OF 1-21, FUNCTION, SUBUNIT. Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein. Kamitani T., Kito K., Nguyen H.P., Yeh E.T.H.J. Biol. Chem. 272:28557-28562(1997) [PubMed] [Europe PMC] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, CLEAVAGE SITE. Cleavage of the C-terminus of NEDD8 by UCH-L3. Wada H., Kito K., Caskey L.S., Yeh E.T.H., Kamitani T.Biochem. Biophys. Res. Commun. 251:688-692(1998) [PubMed] [Europe PMC] Cited for: CLEAVAGE BY UCHL3. Covalent modification of all members of human cullin family proteins by NEDD8. Hori T., Osaka F., Chiba T., Miyamoto C., Okabayashi K., Shimbara N., Kato S., Tanaka K.Oncogene 18:6829-6834(1999) [PubMed] [Europe PMC] Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY. NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression. Kito K., Yeh E.T.H., Kamitani T.J. Biol. Chem. 276:20603-20609(2001) [PubMed] [Europe PMC] Cited for: INTERACTION WITH NUB1. The NEDD8 pathway is essential for SCF(beta -TrCP)-mediated ubiquitination and processing of the NF-kappa B precursor p105. Amir R.E., Iwai K., Ciechanover A.J. Biol. Chem. 277:23253-23259(2002) [PubMed] [Europe PMC] Cited for: FUNCTION. Interaction with Nedd8, a ubiquitin-like protein, enhances the transcriptional activity of the aryl hydrocarbon receptor. Antenos M., Casper R.F., Brown T.J.J. Biol. Chem. 277:44028-44034(2002) [PubMed] [Europe PMC] Cited for: INTERACTION WITH AHR.