PIP5KIII Polyclonal Antibody, 100μg, (ATB-T3733)

Background: Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, May 2010]Product datasheet: Overview Product Description PIP5KIII Polyclonal Antibody, 100μg, (ATB-T3733) Species Reactivities Human,Mouse,Rat Immunogen Synthesized peptide derived from human PIP5KIII around the non-phosphorylation site of S307. Properties Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage Instructions-20°C/1 year References: A novel PIKFYVE mutation in fleck corneal dystrophy. Kotoulas A, et al. Mol Vis, 2011. PMID 22065932 Free PMC Article PIKfyve regulation of endosome-linked pathways. de Lartigue J, et al. Traffic, 2009 Jul. PMID 19582903 Free PMC Article Elevated levels of PtdIns5P in NPM-ALK transformed cells: implication of PIKfyve. Coronas S, et al. Biochem Biophys Res Commun, 2008 Jul 25. PMID 18501703 PIKfyve: Partners, significance, debates and paradoxes. Shisheva A. Cell Biol Int, 2008 Jun. PMID 18304842 Free PMC Article The mammalian phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) regulates endosome-to-TGN retrograde transport. Rutherford AC, et al. J Cell Sci, 2006 Oct 1. PMID 16954148 Free PMC Article