Phospho-A-Raf (S299) Polyclonal Antibody, 100µg, (ATB-P1037)

Background: This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.Product datasheet: Overview Product Description Phospho-A-Raf (S299) Polyclonal Antibody, 100µg, (ATB-P1037) Species Reactivities Human,Mouse,Rat Immunogen Synthesized peptide derived from human A-Raf around the phosphorylation site of S299. Properties Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage Instructions-20°C/1 year Clonality Polyclonal References: Somatic activating ARAF mutations in Langerhans cell histiocytosis. Nelson DS, et al. Blood, 2014 May 15. PMID 24652991 Positive regulation of A-RAF by phosphorylation of isoform-specific hinge segment and identification of novel phosphorylation sites. Baljuls A, et al. J Biol Chem, 2008 Oct 3. PMID 18662992 Identification of key residues in the A-Raf kinase important for phosphoinositide lipid binding specificity. Johnson LM, et al. Biochemistry, 2005 Mar 8. PMID 15736953 Pks, a raf-related sequence in humans. Mark GE, et al. Proc Natl Acad Sci U S A, 1986 Sep. PMID 3529082 Free PMC Article The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus. Beck TW, et al. Nucleic Acids Res, 1987 Jan 26. PMID 3029685 Free PMC Article The complete coding sequence of the human A-raf-1 oncogene and transforming activity of a human A-raf carrying retrovirus. Beck T.W., Huleihel M., Gunnell M., Bonner T.I., Rapp U.R.Nucleic Acids Res. 15:595-609(1987) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1). Lee J.-E., Beck T.W., Brennscheidt U., DeGennaro L.J., Rapp U.R.Genomics 20:43-55(1994) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta. DA-Raf1, a competent intrinsic dominant-negative antagonist of the Ras-ERK pathway, is required for myogenic differentiation. Yokoyama T., Takano K., Yoshida A., Katada F., Sun P., Takenawa T., Andoh T., Endo T.J. Cell Biol. 177:781-793(2007) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION (ISOFORM 2), ALTERNATIVE SPLICING. Cloning of human full-length CDSs in BD Creator(TM) system donor vector. Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Full-length cDNA libraries and normalization. Li W.B., Gruber C., Jessee J., Polayes D.Submitted (APR-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta.