Phospho-ACAP1 (S554) Polyclonal Antibody, 100µg, (ATB-P1017)

Background: GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.Product datasheet: Overview Product Description Phospho-ACAP1 (S554) Polyclonal Antibody, 100µg, (ATB-P1017) Species Reactivities Human Immunogen Synthesized peptide derived from human ACAP1 around the phosphorylation site of S554. Properties Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage Instructions-20°C/1 year Clonality Polyclonal References: Gene and protein expression of centaurin beta 1 (CENTB1) are up-regulated in patients with ulcerative colitis. Yamamoto-Furusho JK, et al. J Crohns Colitis, 2013 Jul. PMID 23317930 Mechanistic insights into regulated cargo binding by ACAP1 protein. Bai M, et al. J Biol Chem, 2012 Aug 17. PMID 22645133 Free PMC Article An ACAP1-containing clathrin coat complex for endocytic recycling. Li J, et al. J Cell Biol, 2007 Jul 30. PMID 17664335 Free PMC Article The Arf6 GTPase-activating proteins ARAP2 and ACAP1 define distinct endosomal compartments that regulate integrin α5β1 traffic. Chen PW, et al. J Biol Chem, 2014 Oct 31. PMID 25225293 Cooperation of phosphoinositides and BAR domain proteins in endosomal tubulation. Shinozaki-Narikawa N, et al. Traffic, 2006 Nov. PMID 17010122 Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1. Nomura N., Nagase T., Miyajima N., Sazuka T., Tanaka A., Sato S., Seki N., Kawarabayasi Y., Ishikawa K., Tabata S.DNA Res. 1:223-229(1994) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow. Cloning of human full-length CDSs in BD Creator(TM) system donor vector. Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph. ACAPs are arf6 GTPase-activating proteins that function in the cell periphery. Jackson T.R., Brown F.D., Nie Z., Miura K., Foroni L., Sun J., Hsu V.W., Donaldson J.G., Randazzo P.A.J. Cell Biol. 151:627-638(2000) [PubMed] [Europe PMC] Cited for: FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-448. Phosphorylation of ACAP1 by Akt regulates the stimulation-dependent recycling of integrin beta1 to control cell migration. Li J., Ballif B.A., Powelka A.M., Dai J., Gygi S.P., Hsu V.W.Dev. Cell 9:663-673(2005) [PubMed] [Europe PMC] Cited for: FUNCTION, PHOSPHORYLATION AT SER-554, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH ITGB1, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-14; SER-29; SER-277; THR-289; SER-358; THR-389; THR-461; SER-554; SER-568; THR-711; TYR-712 AND SER-724.