Phospho-Cdc6 (S54) Polyclonal Antibody, 100µg, (ATB-P0623)

Background: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.Product datasheet: Overview Product Description Phospho-Cdc6 (S54) Polyclonal Antibody, 100µg, (ATB-P0623) Image Species Reactivities Human,Mouse,Monkey Immunogen Synthesized peptide derived from human Cdc6 around the phosphorylation site of S54. Properties Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage Instructions-20°C/1 year Clonality Polyclonal References: PIP-box-mediated degradation prohibits re-accumulation of Cdc6 during S phase. Clijsters L, et al. J Cell Sci, 2014 Mar 15. PMID 24434580 Destabilization of CDC6 upon DNA damage is dependent on neddylation but independent of Cullin E3 ligases. Tan CY, et al. Int J Biochem Cell Biol, 2013 Jul. PMID 23597704 Phosphorylation of Cdc6 at serine 74, but not at serine 106, drives translocation of Cdc6 to the cytoplasm. Yim H, et al. J Cell Physiol, 2013 Jun. PMID 23129444 Knockdown of Cdc6 inhibits proliferation of tongue squamous cell carcinoma Tca8113 cells. Feng CJ, et al. Technol Cancer Res Treat, 2013 Apr. PMID 22974333 Cdc6: a trifunctional AAA+ ATPase that plays a central role in controlling the G(1)-S transition and cell survival. Okayama H. J Biochem, 2012 Oct. PMID 22908236 A human protein related to yeast Cdc6p. Williams R.S., Shohet R.V., Stillman B.Proc. Natl. Acad. Sci. U.S.A. 94:142-147(1997) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Human CDC6/Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phase. Saha P., Chen J., Thome K.C., Lawlis S.J., Hou Z.H., Hendricks M., Parvin J.D., Dutta A.Mol. Cell. Biol. 18:2758-2767(1998) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH PCNA; ORC1 AND CYCLIN-CDK. NIEHS SNPs programSubmitted (SEP-2002) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-238; ASN-295; MET-299; HIS-378 AND ILE-441. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ILE-441. Tissue: Brain. Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage. Hall J.R., Kow E., Nevis K.R., Lu C.K., Luce K.S., Zhong Q., Cook J.G.Mol. Biol. Cell 18:3340-3350(2007) [PubMed] [Europe PMC] Cited for: INTERACTION WITH HUWE1.