Phospho-EF-2 (T56) Polyclonal Antibody, 100 µg, (ATB-P0870)

Background: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.Product datasheet: Overview Product Description Species Reactivities Human,Mouse,Rat Immunogen Synthesized peptide derived from human EF-2 around the phosphorylation site of T56. Properties Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage Instructions-20°C/1 year Clonality Polyclonal References: Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells. Rapp G., Klaudiny J., Hagendorff G., Luck M.R., Heinz K.Biol. Chem. Hoppe-Seyler 370:1071-1075(1989) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary. Construction of a plasmid containing the complete coding region of human elongation factor 2. Hanes J., Freudenstein J., Rapp G., Scheit K.H.Biol. Chem. Hoppe-Seyler 373:201-204(1992) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Ustek D., Bektas M., Cakiris A., Oku B., Bermek E.Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peripheral blood. Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. Cloning and sequence analysis of a cDNA from human ovarian granulosa cells encoding the C-terminal part of human elongation factor 2. Rapp G., Mucha J., Einspanier R., Luck M., Scheit K.H.Biol. Chem. Hoppe-Seyler 369:247-250(1988) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 501-858. Lubec G., Vishwanath V.Submitted (MAR-2007) to UniProtKBCited for: PROTEIN SEQUENCE OF 796-801, IDENTIFICATION BY MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell. Bienvenut W.V.Submitted (AUG-2001) to UniProtKBCited for: CLEAVAGE OF INITIATOR METHIONINE. Proteomic identification of proteins conjugated to ISG15 in mouse and human cells. Giannakopoulos N.V., Luo J.K., Papov V., Zou W., Lenschow D.J., Jacobs B.S., Borden E.C., Li J., Virgin H.W., Zhang D.E.Biochem. Biophys. Res. Commun. 336:496-506(2005) [PubMed] [Europe PMC] Cited for: ISGYLATION. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.Cell 127:635-648(2006) [PubMed] [Europe PMC] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.