Phospho-p21 (T145) Polyclonal Antibody, 100 µg, (ATB-P0200)

Background: May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.Product datasheet: Overview Product Description Species Reactivities Human,Mouse,Rat Immunogen Synthesized peptide derived from human p21 around the phosphorylation site of T145. Properties Form Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage Instructions-20°C/1 year Clonality Polyclonal References: The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Harper J.W., Adami G.R., Wei N., Keyomarsi K., Elledge S.J.Cell 75:805-816(1993) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION. WAF1, a potential mediator of p53 tumor suppression. El-Deiry W.S., Tokino T., Velculescu V.E., Levy D.B., Parsons R., Trent J.M., Lin D., Mercer W.E., Kinzler K.W., Vogelstein B.Cell 75:817-825(1993) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. p21 is a universal inhibitor of cyclin kinases. Xiong Y., Hannon G.J., Zhang H., Casso D., Kobayashi R., Beach D.Nature 366:701-704(1993) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Use of a sensitive and efficient subtraction hybridization protocol for the identification of genes differentially regulated during the induction of differentiation in human melanoma cells. Jiang H., Fisher P.B.Mol. Cell. Differ. 1:285-299(1993)Cited for: NUCLEOTIDE SEQUENCE [MRNA]. The melanoma differentiation-associated gene mda-6, which encodes the cyclin-dependent kinase inhibitor p21, is differentially expressed during growth, differentiation and progression in human melanoma cells. Jiang H., Lin J., Su Z.Z., Herlyn M., Kerbel R.S., Weissman B.E., Welch D.R., Fisher P.B.Oncogene 10:1855-1864(1995) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen. Noda A., Ning Y., Venable S.F., Pereira-Smith O.M., Smith J.R.Exp. Cell Res. 211:90-98(1994) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Two variants of the CIP1/WAF1 gene occur together and are associated with human cancer. Mousses S., Oezcelik H., Lee P.D., Malkin D., Bull S.B., Andrulis I.L.Hum. Mol. Genet. 4:1089-1092(1995) [PubMed] [Europe PMC] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-31. NIEHS SNPs programSubmitted (APR-2002) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-31. Cloning of human full-length CDSs in BD Creator(TM) system donor vector. Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201). Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databasesCited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].